Marker Overview
NameNem_Aradu.A04_114769893
Marker TypeSNP
Accessionn/a
OrganismArachis spp. (multiple peanut species)
Source Description
Primer1 Sequence
>Nem_Aradu.A04_114769893_Fwd
TCAAGTCGTGTGTTCTCTACACC
Primer2 Sequence
>Nem_Aradu.A04_114769893_Rev_FAM
GAAGGTGACCAAGTTCATGCTTCTTGTGACATGAGCTACAACTTCT
Primer3 Sequence
>Nem_Aradu.A04_114769893_Rev_VIC
GAAGGTCGGAGTCAACGGATTTTGTGACATGAGCTACAACTTCG
Genetic Map(s)This marker has not been placed on any genetic maps
Publication(s)Primary: Leal-Bertioli, Moretzsohn et al., 2016a
CommentsThe same PCR amplification pattern was observed for Arachis stenosperma, (A. batizocoi x A. stenosperma)4x, (A. gregoryi x A. stenosperma)4x, A. duranensis, A. hypogaea, and A. monticola. Nem_Aradu.A04_114769893_Fwd is an allele flanking forward primer, Nem_Aradu.A04_114769893_Rev_FAM is an allele-specific reverse primer that detects SNP allele C, and Nem_Aradu.A04_114769893_Rev_VIC is an allele-specific reverse primer that detects SNP allele A. The SNP is specifically a Single Nucleotide Polymorphism Kompetitive Allele Specific Polymerase Chain Reaction (SNP-KASP) marker. This marker is associated with the A. stenosperma-derived Root Knot Nematode resistance locus on linkage group A04 and was validated in a tetraploid context. Nem_Aradu.A04_114769893 was developed using information taken from the A. duranensis genome sequence.
Sequenceunknown
Marker Positions
NameNem_Aradu.A04_114769893
Physical position(s)physical position unknown
Map Position(s)This marker is not placed on any map.