Overview
Map NameTT_Huayu28_x_P76_a
Download (CMap format)download
Publication Map NameHuayu28_x_P76 high-density genetic map
Map UnitscM
Map viewcmap-js
SpeciesArachis hypogaea
Mapping populationTT_Huayu28_x_P76_a
Parent 1Huayu28
Parent 2P76
Population Size146
Population TypeF2:11 RIL
MethodsHighMap
DescriptionA mapping population of 146 F2:11 recombinant inbred lines (RILs) was produced A1:P2 a cross of the cultivated peanut (Arachis hypogaea L.) maternal inbred line 'Huayu28' and paternal inbred line 'P76'. The high-resolution genetic linkage map is composed of 2,334 markers (2,266 SNPs and 68 microsatellites) placed upon 20 linkage groups. The total length of the map is 2,586.37 cM; lengths of linkage groups range from 68.79 to 191.24 cM. The mean distance between adjacent markers is 2.25 cM for the whole map, whereas the average inter-marker distances for individual linkage groups range from 0.3 to 6.09 cM. The number of markers per linkage group ranges from 13 to 369, with the average count of markers on each linkage group being 116.7. Seven hundred of the 2,334 mapped markers across 14 linkage groups exhibited significant segregation distortion, particularly on linkage groups A04, B03, and B05. This high-density map is the first to be developed in cultivated peanut incorporating de novo polymorphic SNP markers discovered using a SLAF-seq (Specific Length Amplified Fragment Sequencing) methodology. The authors first developed 433,679 high-quality SLAFs and then discovered that 29,075 of these SNP markers were polymorphic. There were 7,949 polymorphic markers that followed an aa_x_bb segregation pattern appropriate for linkage analysis, out of which 2,266 SNPs were linked together in the final map.
PublicationHu, Zhang et al., 2018a: High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs.
CommentThe peanut variety 'Huayu28' demonstrates early-maturity, normal oleic acid content of oil, and small-sized seeds. 'P76' is a variety of peanut characterized by late-maturity, high oleic acid content of oil, and medium-sized seeds. In 2014 seedlings of 'Huayu28', 'P76', and the 146 F2:11 RILs were grown in the field at Shandong Peanut Research Institute in Laixi, Shandong Province, China. Total genomic DNA was extracted from young leaves of the parents and progeny to produce SLAF-seq SNP markers and ultimately the molecular linkage map.