Map NameTT_RunnerIAC886_x_A.batizocoi-A.stenosperma_a
Download (CMap format)not available
Publication Map NameRunnerIAC886_x_BatSten1 high-density linkage map for the RBS-F2 population
Map UnitscM
Map viewcmap-js
SpeciesArachis spp.
Mapping populationunknown
Parent 1unknown
Parent 2unknown
Population Size194
Population TypeF2
MethodsJoinMap (v. 4.0)
DescriptionThis high-density, SNP-based genetic map was created using a 194-member RBS-F2 segregating population derived by selfing an F1 hybrid progeny, which was itself taken from a cross between the cultivated peanut Arachis hypogaea cv. 'Runner IAC 886' and the synthetic allotetraploid BatSten1. The induced BatSten1, developed to introgress diploid-derived nematode resistance into tetraploid contexts, was produced by mating the wild peanut accessions Arachis stenosperma PI666100 'V10309' (A genome) and Arachis batizocoi PI298639 'K9484' (K-genome: B-genome in the broad sense). The linkage map incorporates 1499 SNP markers into 20 linkage groups; the 10 A-subgenome linkage groups are based on markers derived from A. stenosperma whereas the 10 K-subgenome linkage groups are based on markers derived from A. batizocoi. The marker number per linkage group ranged from 36 (B04) to 217 (A01). The total map distance is 3984.9 cM and individual linkage groups ranged in length from 100.7 cM (A05) to 359.5 cM (A02). The average distance between adjacent markers varied from 1.35 cM (A01) to 3.8 cM (A06) and the largest inter-marker distance was observed on linkage group A06 with 29.48 cM.
PublicationBallen-Taborda, Chu et al., 2019a: A new source of root-knot nematode resistance from Arachis stenosperma incorporated into allotetraploid peanut (Arachis hypogaea).
CommentArachis stenosperma V10309, Arachis batizocoi K9484, and BatSten1 (A. batizocoi K9484 x A. stenosperma V10309)4x showed resistance to the Root-knot nematode (no or small galls and low egg numbers). The Arachis hypogaea cultivar 'Runner IAC 886' was susceptible to Root-knot nematode infection and pathology. The induced allotetraploid BatSten1 has 40 total chromosomes (2n=4x=40). Although the members of the RBS-F2 progeny population exhibited variable amounts of resistance to Meloidogyne arenaria, on the whole values of EGR, RF, and GI traits were biased in favor of resistance. The F2 population that was genotyped originally comprised 196 F2 progeny, but 2 individuals were eliminated because of excessive missing data points. They discovered 1587 total polymorphic SNPs, of which 911 were associated with the A-subgenome (A. stenosperma) and 676 with the B/K-subgenome (A. batizocoi); 1499 of these SNPs were subsequently incorporated into the genetic linkage map. Physical positions of A. stenosperma markers were assessed based on positions of their homologues on the A. duranensis pseudomolecules and positions of A. batizocoi markers were determined using pseudomolecules of A. ipaensis. Vegetative propagation was used to keep the RBS-F2 population alive in the greenhouse for phenotyping over different years. The wild accessions of Arachis were provided by the USDA-GRIN system.