Project NameBurow, Starr et al., 2014a exp1
Introgression of homeologous quantitative trait loci (QTLs) for resistance to the root-knot nematode [Meloidogyne arenaria (Neal) Chitwood] in an advanced backcross-QTL population of peanut (Arachis hypogaea L.)
Additional information about this project:
Property NameValue
QTL Experiment
Project Map CollectionTT_Florunner_x_A.batizocoi-A.cardenasii-A.diogoi_a
Project Comment'As part of an effort to detect small-effect QTLs for root-knot nematode resistance within introgression lines, an advanced backcross population was developed by crossing the cultivated tetraploid peanut line ''Florunner'' (component line UF439-16-10-3-2) with the synthetic amphidiploid TxAG-6 [(A. batizocoi K9484_x_(A. cardenasii GKP10017_x_ A. diogoi GKP10602))4x]. This BC3 population was derived from a 78 member BC1 population that provided the genetic map (Burow, Simpson et al., 2001a) upon which the authors placed the QTLs revealed by the advanced backcross-QTL analysis. The BC1 genetic map (Burow, Simpson et al., 2001a) is thus the source of the RFLP markers used to genotype the BC3 population and established the cM distances between markers. These BC1 progeny were then backcrossed to ''Florunner'' to form a BC2 population; each resulting BC2 line was again backcrossed to ''Florunner'' to produce up to 10 BC3 plants per BC2 line (mean of 5.7 BC3 progeny per BC2 line). Out of the original 78 BC1 families described in Burow, Simpson et al. (2001a), the descendents of 41 of them were tested for nematode resistance. All BC3 linkage groups in this study showed evidence of genetic introgression from the TxAG-6 amphidiploid. The 233 BC3 plants were grown in a greenhouse and inoculated with eggs of Meloidogyne arenaria.'