Overview
TitleRecombination is Suppressed in an Alien Introgression in Peanut Harboring Rma, a Dominant Root-Knot Nematode Resistance Gene
AuthorsNagy ED, Chu Y, Guo Y, Khanal S, Tang S, Li Y, Dong WB, Timper P, Taylor C, Ozias-Akins P, Holbrook CC, Beilinson V, Nielsen NC, Stalker HT, Knapp SJ
TypeJournal
Media TitleMolecular Breeding
Volume26
Issue2
Year2010
Page(s)357-370
CitationNagy ED, Chu Y, Guo Y, Khanal S, Tang S, Li Y, Dong WB, Timper P, Taylor C, Ozias-Akins P, Holbrook CC, Beilinson V, Nielsen NC, Stalker HT, Knapp SJ. (2010). Recombination is Suppressed in an Alien Introgression in Peanut Harboring Rma, a Dominant Root-Knot Nematode Resistance Gene. Molecular Breeding. 26:2 357-370

Abstract

Rma, a dominant root-knot nematode resistance gene introduced into tetraploid peanut (Arachis hypogaea) from a synthetic allotetraploid donor (TxAG-6), has been widely deployed in modern cultivars. The genomic location and borders of the alien chromosome segment introgressed from TxAG-6 into NemaTAM (a BC7-derived introgression line) and other modern cultivars carrying Rma have not been genetically mapped, and resistance gene candidates (RGCs) have not been identified for Rma. Our study focused on densely populating the alien introgression with codominant DNA markers, identifying and mapping the borders of the alien introgression carried by NemaTAM, and identifying RGCs for Rma. Altogether, 2,847 simple sequence repeat (SSR) and 380 single strand conformational polymorphism (SSCP) markers were screened for linkage to Rma-247 of the SSCP markers targeted 202 nucleotide binding site (NBS) leucine-rich repeat (LRR) and other resistance (R) gene homologs (75 were identified by mining a peanut EST database). SSR, NBS-LRR, and Ser/Thr receptor-like protein loci within the alien introgression co-segregated with Rma in an F4 population (Gregory 9 Tifguard) and were tightly linked and spanned 3.4 cM in an F5 population (NemaTAM 9 GP-NC-WS-14). By comparative mapping in the A-genome progenitor of peanut (A. duranensis), Rma was discovered to have been introduced on an interstitial alien chromosome segment spanning one-third to one-half of chromosome 9A. Numerous codominant DNA markers were identified for finer mapping of Rma, shortening the alien introgression harboring Rma by marker-assisted selection, and introducing novel root-knot nematode R-genes into peanut by targeting syntenic segments on chromosomes 9A and 9B in wild diploid donors.
Features
This publication contains information about 476 features:
Feature NameUniquenameType
RGC132cRGC132cgenetic_marker
RGC135RGC135genetic_marker
RGC137aRGC137agenetic_marker
RGC137bRGC137bgenetic_marker
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RGC140aRGC140agenetic_marker
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RGC141RGC141genetic_marker
RGC144aRGC144agenetic_marker
RGC144bRGC144bgenetic_marker
RGC146aRGC146agenetic_marker
RGC146bRGC146bgenetic_marker
RGC150aRGC150agenetic_marker
RGC150bRGC150bgenetic_marker
RGC152aRGC152agenetic_marker
RGC152bRGC152bgenetic_marker
RGC154RGC154genetic_marker
RGC158aRGC158agenetic_marker
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RGC160aRGC160agenetic_marker
RGC160bRGC160bgenetic_marker
RGC160cRGC160cgenetic_marker
RGC162RGC162genetic_marker
RGC164RGC164genetic_marker
RGC165RGC165genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s11032-010-9430-4
KeywordsArachis, Meloidogyne, Marker-assisted selection, Fabaceae, Nucleotide binding site leucine-rich repeat, Receptor-like kinase
Cross References
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DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s11032-010-9430-4