Overview
TitleRecombination is Suppressed in an Alien Introgression in Peanut Harboring Rma, a Dominant Root-Knot Nematode Resistance Gene
AuthorsNagy ED, Chu Y, Guo Y, Khanal S, Tang S, Li Y, Dong WB, Timper P, Taylor C, Ozias-Akins P, Holbrook CC, Beilinson V, Nielsen NC, Stalker HT, Knapp SJ
TypeJournal
Media TitleMolecular Breeding
Volume26
Issue2
Year2010
Page(s)357-370
CitationNagy ED, Chu Y, Guo Y, Khanal S, Tang S, Li Y, Dong WB, Timper P, Taylor C, Ozias-Akins P, Holbrook CC, Beilinson V, Nielsen NC, Stalker HT, Knapp SJ. (2010). Recombination is Suppressed in an Alien Introgression in Peanut Harboring Rma, a Dominant Root-Knot Nematode Resistance Gene. Molecular Breeding. 26:2 357-370

Abstract

Rma, a dominant root-knot nematode resistance gene introduced into tetraploid peanut (Arachis hypogaea) from a synthetic allotetraploid donor (TxAG-6), has been widely deployed in modern cultivars. The genomic location and borders of the alien chromosome segment introgressed from TxAG-6 into NemaTAM (a BC7-derived introgression line) and other modern cultivars carrying Rma have not been genetically mapped, and resistance gene candidates (RGCs) have not been identified for Rma. Our study focused on densely populating the alien introgression with codominant DNA markers, identifying and mapping the borders of the alien introgression carried by NemaTAM, and identifying RGCs for Rma. Altogether, 2,847 simple sequence repeat (SSR) and 380 single strand conformational polymorphism (SSCP) markers were screened for linkage to Rma-247 of the SSCP markers targeted 202 nucleotide binding site (NBS) leucine-rich repeat (LRR) and other resistance (R) gene homologs (75 were identified by mining a peanut EST database). SSR, NBS-LRR, and Ser/Thr receptor-like protein loci within the alien introgression co-segregated with Rma in an F4 population (Gregory 9 Tifguard) and were tightly linked and spanned 3.4 cM in an F5 population (NemaTAM 9 GP-NC-WS-14). By comparative mapping in the A-genome progenitor of peanut (A. duranensis), Rma was discovered to have been introduced on an interstitial alien chromosome segment spanning one-third to one-half of chromosome 9A. Numerous codominant DNA markers were identified for finer mapping of Rma, shortening the alien introgression harboring Rma by marker-assisted selection, and introducing novel root-knot nematode R-genes into peanut by targeting syntenic segments on chromosomes 9A and 9B in wild diploid donors.
Features
This publication contains information about 476 features:
Feature NameUniquenameType
R2430E-2R2430E-2genetic_marker
R2430E-3R2430E-3genetic_marker
R2430E-4R2430E-4genetic_marker
RGC001aRGC001agenetic_marker
RGC001bRGC001bgenetic_marker
RGC004aRGC004agenetic_marker
RGC004bRGC004bgenetic_marker
RGC005aRGC005agenetic_marker
RGC005bRGC005bgenetic_marker
RGC006aRGC006agenetic_marker
RGC006bRGC006bgenetic_marker
RGC007RGC007genetic_marker
RGC008aRGC008agenetic_marker
RGC008bRGC008bgenetic_marker
RGC013RGC013genetic_marker
RGC017aRGC017agenetic_marker
RGC017bRGC017bgenetic_marker
RGC018aRGC018agenetic_marker
RGC018bRGC018bgenetic_marker
RGC021RGC021genetic_marker
RGC024RGC024genetic_marker
RGC028RGC028genetic_marker
RGC029RGC029genetic_marker
RGC032RGC032genetic_marker
RGC033RGC033genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s11032-010-9430-4
KeywordsArachis, Meloidogyne, Marker-assisted selection, Fabaceae, Nucleotide binding site leucine-rich repeat, Receptor-like kinase
Cross References
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DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s11032-010-9430-4