Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0001AhTE0001genetic_marker
AhTE0002AhTE0002genetic_marker
AhTE0003AhTE0003genetic_marker
AhTE0004AhTE0004genetic_marker
AhTE0005AhTE0005genetic_marker
AhTE0006AhTE0006genetic_marker
AhTE0007AhTE0007genetic_marker
AhTE0008AhTE0008genetic_marker
AhTE0009AhTE0009genetic_marker
AhTE0010AhTE0010genetic_marker
AhTE0011AhTE0011genetic_marker
AhTE0012AhTE0012genetic_marker
AhTE0013AhTE0013genetic_marker
AhTE0014AhTE0014genetic_marker
AhTE0015AhTE0015genetic_marker
AhTE0016AhTE0016genetic_marker
AhTE0017AhTE0017genetic_marker
AhTE0018AhTE0018genetic_marker
AhTE0019AhTE0019genetic_marker
AhTE0020AhTE0020genetic_marker
AhTE0021AhTE0021genetic_marker
AhTE0022AhTE0022genetic_marker
AhTE0023AhTE0023genetic_marker
AhTE0024AhTE0024genetic_marker
AhTE0025AhTE0025genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055