Overview
TitleHigh-Density Genetic Map Using Whole-Genome Resequencing for Fine Mapping and Candidate Gene Discovery for Disease Resistance in Peanut
AuthorsAgarwal G, Clevenger J, Pandey MK, Wang H, Shasidhar Y, Chu Y, Fountain JC, Choudhary D, Culbreath AK, Liu X, Huang G, Wang X, Deshmukh R, Holbrook CC, Bertioli DJ, Ozias-Akins P, Jackson SA, Varshney RK, Guo B
TypeJournal
Media TitlePlant Biotechnology Journal
VolumeN/A
IssueN/A
Year2018
Page(s)N/A
CitationAgarwal G, Clevenger J, Pandey MK, Wang H, Shasidhar Y, Chu Y, Fountain JC, Choudhary D, Culbreath AK, Liu X, Huang G, Wang X, Deshmukh R, Holbrook CC, Bertioli DJ, Ozias-Akins P, Jackson SA, Varshney RK, Guo B. (2018). High-Density Genetic Map Using Whole-Genome Resequencing for Fine Mapping and Candidate Gene Discovery for Disease Resistance in Peanut. Plant Biotechnology Journal. NULL:NULL NULL

Abstract

Whole-genome resequencing (WGRS) of mapping populations has facilitated development of high-density genetic maps essential for fine mapping and candidate gene discovery for traits of interest in crop species. Leaf spots, including early leaf spot (ELS) and late leaf spot (LLS), and Tomato spotted wilt virus (TSWV) are devastating diseases in peanut causing significant yield loss. We generated WGRS data on a recombinant inbred line population, developed a SNP-based high-density genetic map, and conducted fine mapping, candidate gene discovery and marker validation for ELS, LLS and TSWV. The first sequence-based high-density map was constructed with 8869 SNPs assigned to 20 linkage groups, representing 20 chromosomes, for the 'T' population (Tifrunner x GT-C20) with a map length of 3120 cM and an average distance of 1.45 cM. The quantitative trait locus (QTL) analysis using high-density genetic map and multiple season phenotyping data identified 35 main-effect QTLs with phenotypic variation explained (PVE) from 6.32% to 47.63%. Among major-effect QTLs mapped, there were two QTLs for ELS on B05 with 47.42% PVE and B03 with 47.38% PVE, two QTLs for LLS on A05 with 47.63% and B03 with 34.03% PVE and one QTL for TSWV on B09 with 40.71% PVE. The epistasis and environment interaction analyses identified significant environmental effects on these traits. The identified QTL regions had disease resistance genes including R-genes and transcription factors. KASP markers were developed for major QTLs and validated in the population and are ready for further deployment in genomics-assisted breeding in peanut.
Maps
This publication contains information about 1 maps:
Map Name
TT_Tifrunner_x_GT-C20_c
Features
This publication contains information about 7,220 features:
Feature NameUniquenameType
A01_304818A01_304818genetic_marker
A01_305650A01_305650genetic_marker
A01_313131A01_313131genetic_marker
A01_313351A01_313351genetic_marker
A01_319619A01_319619genetic_marker
A01_319819A01_319819genetic_marker
A01_830764A01_830764genetic_marker
A01_859822A01_859822genetic_marker
A01_879272A01_879272genetic_marker
A01_915776A01_915776genetic_marker
A01_929082A01_929082genetic_marker
A01_948500A01_948500genetic_marker
A01_968029A01_968029genetic_marker
A01_1108096A01_1108096genetic_marker
A01_1109257A01_1109257genetic_marker
A01_1409478A01_1409478genetic_marker
A01_1409500A01_1409500genetic_marker
A01_1413815A01_1413815genetic_marker
A01_1419679A01_1419679genetic_marker
A01_1419688A01_1419688genetic_marker
A01_1419700A01_1419700genetic_marker
A01_1433415A01_1433415genetic_marker
A01_1473214A01_1473214genetic_marker
A01_1734811A01_1734811genetic_marker
A01_1734989A01_1734989genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttps://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.12930
KeywordsWhole-genome resequencing, High-density genetic map, Quantitative trait loci, Early leaf spot, Late leaf spot, Tomato spotted wilt virus
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1111/pbi.12930
PMID: PubMedPMID:29637729
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenaemType
TT_Tifrunner_x_GT-C20_cTT_Tifrunner_x_GT-C20_cCultivar